ABSTRACT
ObjectiveTo analyze the correlation between the changes of swallowing function and aspiration in stroke patients using videofluroscopic swallowing study. MethodsFrom January, 2014 to December, 2021, 240 stroke patients who underwent videofluroscopic swallowing study in the radiology department of Xuzhou Central Hospital were retrospectively analyzed. They were divided into aspiration group (n = 108, ≥ grade 5) and non-aspiration group (n = 132, < grade 5) according to Rosenbek Penetration/Aspiration Scale (PAS). The swallowing function changes in the oral and pharyngeal phases were compared between two groups according to Videofluoroscopic Dysphagia Scale, the risk factors of aspiration were analyzed using Logistic multivariate regression. ResultsThere were significant differences in incomplete lip closure, impaired bolus formation, apraxia, incomplete tongue-to-palate contact, premature bolus loss, prolonged oral transit time, delayed triggering of pharyngeal swallow, epiglottis folding dysfunction, pyriform sinus residue, and coating of pharyngeal wall (χ2 > 5.802, P < 0.05). Prolonged oral transit time (OR = 3.964, P = 0.036), delayed triggering of pharyngeal swallow (OR = 5.684, P < 0.001), epiglottis folding dysfunction (OR = 11.571, P < 0.001) and pyriform sinus residue (OR = 4.524, P = 0.002) were correlated with aspiration. ConclusionProlonged oral transit time, delayed triggering of pharyngeal swallow, epiglottis folding dysfunction and pyriform sinus residue are risk factors of aspiration after stroke.
ABSTRACT
Objective@#: To explore the correlation between the polymorphism of histone deacetylase 9 gene (rs1060499865, rs723296, rs957960) and ischemic stroke (IS) in Chinese Han population in Dali region. @*Methods@#: This study included 155 IS patients and 128 healthy physical examinees. TaqMan-polymerase chain reaction technology and multivariate logistic regression were performed. @*Results@#: In the case group, there was no polymorphism of rs1060499865 observed in the two groups; whereas on the rs723296 locus the frequencies of C allele and TC genotype were significantly higher than that in the control group, alleles C and T were associated with a 2.158-fold increase in IS risk, and genotypes TC and TT were associated with a 2.269-fold increase in IS risk. The locus rs957960 exhibited no significant difference between the two groups. @*Conclusion@#: An association between rs723296 and the risk of IS was found in the Chinese Han population in Dali region. No significant association was found between rs1060499865, rs957960 and IS in the Chinese Han population in Dali region.
ABSTRACT
To perform a systematic review of the data collected from case-control studies conducted earlier to investigate the correlation between E-selectin S128R polymorphism and ischemic stroke (IS) risk among the Chinese population. The PubMed, Web of Science, Chinese biomedical literature database (CBM), Chinese databases China National Knowledge Infrastructure (CNKI), WanfangData knowledge service platform (Wanfang Data), and information resource integration service platform (VIP) Databases were searched to retrieve case-control studies on the correlation between E-selectin gene S128R polymorphism and IS from the inception of the database till June 2019. The literature was screened, data were extracted, the risk of bias was reviewed, and the studies included were assessed independently by two reviewers. Stata ver. 12.0 software (Stata Corp LLC, College Station, TX, USA) was used to perform the meta-analysis. A total of 2907 cases from eight case-control studies involving 1478 IS patients and 1429 controls were included in this study. The R allele and RS genotype in E-selectin were found to be associated with the risk of IS as per the results of the meta-analysis (R vs. S : odds ratio [OR], 2.75; 95% confidence interval [CI], 2.15-3.51; p<0.00001; RS vs. SS : OR, 2.50; 95% CI, 1.95-3.19; p<0.00001; RR+RS vs. SS : OR, 2.85, 95% CI, 2.21-3.67; p<0.00001). The E-selectin gene S128R polymorphism is likely related to IS based on the results of a meta-analysis in the Chinese population, and the R allele and RS genotype of E-selectin may be IS risk factors.
ABSTRACT
Objective To investigate the neuroprotective effects of Fasudil in cerebral I/R injury in mice.Methods 51 C57BL/6J mice was divided into two groups,CMC treated group (n=26) and Fasudil treated group (n=25),randomly.The mice were treated with Fasudil (10 mg/kg) or CMC (0.5％ CMC 10 ml/kg) separately.Then the treated mice were subjected to 60 min of focal ischemia and 18 h reperfusion.The infarct volume of brain was analyzed by TTC staining with MCID image system.BBB permeability was assessed by Evans blue extravasation and albumin leakage which was detected by immuno-blotting assay.The activity of MMP9 was analyzed by zymography.Results The infarct volume in CMC group ((99.07±6.53) mm3) was larger than that in Fasudil group ((57.02±8.93) mm3),the difference was statistically significant (P＜0.01).The activity of MMP9 in the mice treated with Fasudil was lower than that in CMC group.Compared with the CMC group(albumin (2.95±0.77),Evans blue (5.15±0.24)),the albumin and Evans blue content in the Fasudil treated group (albumin (1.04±0.18),Evans blue (1.96±0.31))reduced significanctly(all P＜0.01).Conclusion Fasudil protects I/R damage by inhibiting the activity of MMP9 to maintain blood-brain barrier permeability.
ABSTRACT
Objective To evaluate the effects of Acorustatarinowii Schott(ATS)on chronic restraint stress mice and its possible mechanisms. Methods Chronic stressed mice underwent a repeated 8h/d constraint in a specific behavior blocker ,however ,only CSA mice received once gavages of 4.5 g/(kg · d)ATS decoction for con-secutive 28 days. Morris water maze(MWM)task was conducted for evaluating learning and memory of the mice. ELISA was used to examine the levels of plasma corticosteroid. Results ATS dramatically ameliorated cognitive impairments and decreased serum corticosteroid level in the stressed mice(P<0.01). Conclusion ATS improves cognitive deficits provoked by chronic stress in mice ,which may attribute to decreasing plasma corticosteroid levels.
ABSTRACT
Objective To investigate the influence of focal insular glioma on risky decision-making and to verify the causal relationship between insular and risky decision-making cognitive process.Methods 12 patients with focal insular glioma,15 healthy individuals and 15 lesion controls with brain glioma predominantly affecting non-insular lobes were tested with a risky decision-making task.The results were compared by statistical methods.Results The bet time (BT),report time (RT) and bet or report failure (F) of insular glioma patients were (1.61±0.06) s,(1.61±0.10) s and (2(2,3.75)) respectively.BT,RT and F of lesion controls were (0.70±0.11) s,(0.69±0.11s) and (0(0,0)) respectively and those of healthy group were (0.71 ±0.10) s,(0.68 ±0.11) s and (0(0,1)) respectively.Compared with normal subjects and lesion controls,BT and RT of insular glioma patients were significantly extended,and F was observably increased (P＜0.01).BT,RT and F of lesion controls had no significant difference compared with those of healthy group (P＞0.05).Conclusion The risky decision-making cognitive process may be influenced by insular glioma.The insular region plays a necessary role in decision-making under risk and insular causally is involved in risky decision making.
ABSTRACT
Objective To investigate the epidemiological characteristics of patients with craniocerebral injury in primary level hospital of Pearl River Delta and provide evidences for developing reasonable prevention and management policy of this disease. Methods The gender,age,injury time,occupation,injury mechanism, injury information,the relation between the craniocerebral injury and the alcohol drink in the 1 898 patients with craniocerebral injury admitted to Changping People's Hospital of Dongguan in Guangdong Province were analyzed with descriptive epidemiological method. Results There were more male cases with craniocerebral injury than in female ones(1 402 cases vs. 496 cases). The major group of craniocerebral injury consisted of patients aged between 20 to 39 years old〔1088 cases(57.32%)〕. The incidence of the injury in productive workers was higher than that of employees in other kinds of work〔57.48%(1 091 cases)vs. 42.52%(807 cases)〕. The incidences of such cases were the highest between March to May〔28.71%(545 cases)〕and in October〔9.69%(184 cases)〕,December〔11.22%(213 cases)〕than those in other months. The peak days of the incidence in a week were Saturday〔392 cases(20.65%)〕and Sunday〔375 cases(19.77%)〕,but the rate of Thursday was the lowest〔201 cases(10.59%)〕. The peak time in a day was between 18 to 24 o'clock〔961 cases(50.63%)〕. Traffic accident was the leading cause of the injury accounting for 52.95%. The incidence of mild to moderate craniocerebral injury(79.98%)was much higher than that of severe and especially severe injury(20.02%). The alcohol drink was bound up with craniocerebral injury. Conclusion The occurrence of craniocerebral injury has certain regularities concerning the respects of patients' gender, age, occupation,injury time,injury mechanism,types of injury,etc and based on the epidemiological characteristics,a proper management policy should be taken to effectively reduce the morbidity of craniocerebral injury.
ABSTRACT
Objective To investigate the efficacy of damage control surgery for severe abdominal trauma.Methods The clinical data of 82 cases with severe abdominal trauma who received damage control surgery (DCS group) were retrospectively analyzed.The treatment conditions and treatment effect were analyzed.Another 82 cases with abdominal trauma who were not given damage control operation were considered as the control group.Results In DCS group,71 cases improved,the effective rate was 86.6％;11 cases deaths,mortality was 13.4％.4 cases had severe liver rupture,36h after surgery died of multiple organ failure ;3 cases of colon damage,1 d concurrent infections in the postoperative shock and died ;3 cases of retroperitoneal hematoma complicated with coagulopathy,died 20h after operation.In control group,82 cases of abdominal trauma patients after conventional treatment,73 cases improved,and the effective rate was 89.0％ ;9 deaths,mortality was 10.9％.71 patients of DCS group after resuscitation after ICU oxygen saturation,temperature,PH value,PT value and ICU resuscitation significantly improved compared with before treatment,the differences were statistically significant(t =2.467,2.449,2.738,2.453,all P ＜ 0.05),compared with the control group,the differences were not statistically significant (t =1.303,1.312,1.341,1.325,all P ＞ 0.05).Conclusion Damage control surgery for severe abdominal trauma achieved good effect,it is an effective treatment for severe abdominal trauma.
ABSTRACT
Escherichia coli NZN111 is a double mutant with lactate dehydrogenase (ldhA) and pyruvate formate-lyase (pflB) inactivated. Under anaerobic conditions, disequilibrium of coenzyme NADH and NAD+ causes Escherichia coli NZN111 losing the glucose utilizing capability. In this study, we constructed a recombinant strain E. coli NZN111/pTrc99a-mdh and overexpressed the mdh gene with 0.3 mmol/L of IPTG under anaerobic fermentation condition in sealed bottles. The specific malate dehydrogenase (MDH) activity in the recombinant strain was 14.8-fold higher than that in E. coli NZN111. The NADH/ NAD+ ratio decreased from 0.64 to 0.26 and the concentration of NAD+ and NADH increased 1.5-fold and 0.2-fold respectively. Under anaerobic conditions, the recombinant strain possessed the capability of growth and glucose absorption. We took dual-phase fermentation for succinate production. After the dry cell weight (DCW) reached 6.4 g/L under aerobic conditions, the cell culture was changed to anaerobic conditions. After 15 h, 14.75 g/L glucose was consumed and succinic acid reached 15.18 g/L. The yield of succinic acid was 1.03 g/g Glu and the productivity of succinic acid was 1.012 g/(L x h).
Subject(s)
Acetyltransferases , Genetics , Anaerobiosis , Escherichia coli , Genetics , Metabolism , Fermentation , Gene Knockout Techniques , Glucose , Metabolism , L-Lactate Dehydrogenase , Genetics , Malate Dehydrogenase , Genetics , Metabolism , Mutation , Recombinant Proteins , Genetics , Recombination, Genetic , Succinic Acid , MetabolismABSTRACT
Escherichia coli strain NZN111 is a promising candidate for the fermentative production of succinate. However, because lactate dehydrogenase and pyruvate formate lyase were inactivated in NZN111, this strain had an unbalanced NADH/NAD+ ratio and could not use glucose under anaerobic conditions. In this study, a recombinant strain E. coli NZN111/pTrc99a-pncB was constructed to overexpress the nicotinic acid phosphoribosyl transferase gene (pncB). Under anaerobic conditions with the addition of 0.5 mmol/L nicotinic acid and 0.3 mmol/L isopropyl beta-D-thiogalactopyranoside (IPTG), the specific nicotinic acid phosphoribosyl transferase (NAPRTase, EC 2.4.2.11) activity in the recombinant strain was 11-fold higher than that in E. coli NZN111, the concentration of NAD(H) was increased by 3.85-fold, especially the concentration of NAD+ was increased by 5.17-fold and NADH/NAD+ was decreased from 0.640 to 0.125. The recombinant strain regained the capability of growth and glucose utilization under anaerobic conditions.
Subject(s)
Acetyltransferases , Genetics , Metabolism , Anaerobiosis , Escherichia coli , Classification , Genetics , Metabolism , Fermentation , Genetic Enhancement , Methods , Glucose , Metabolism , L-Lactate Dehydrogenase , Genetics , Metabolism , NAD , Metabolism , Nicotinamide Phosphoribosyltransferase , Genetics , Succinic Acid , MetabolismABSTRACT
Objective To investigate effects of different solvent, different temperature and different concentration of hydroxypropyl cyclodextrin on solubility of asarone. Method Determination of asarone solubility in different solvent, at different temperature and different concentration of cyclodextrin were carried out by HPLC-UV method. Results The solubility of asarone in water, tea oil, ethanol, and methanol were 0. 1 mg·mL-1 13.44 mg·mL-1, 1.87 mg·mL-1 and 1.76 mg·mL-1, respectively. The concentration and temperature of cyclodextrin showed a positive correlation with solubility of asarone. At normal temperature, the solubility of asarone in water increased 10 times by 45 % hydroxypropyl cyclodextrin. Conclusion Asarone has poor solubility in water and it can be improved by high concentration of hydroxypropyl cyelodetrin.
ABSTRACT
AIM:The Effects of Spirulina Platensis Polysaccharide (SPP)on peripheral NK cell from healthy persons and acute leukemia patients were studied.METHODS:The activities of NK cell on target cell K 562 was measured with MTT method.RESULTS:The results showed that SPP could argument the NK cell activity from varies peroid cases with acute leukemia.SPP had no activity on NK cell from normal person.Cytotoxicity did not present when the peripheral blood mononuclear cell were co incubated with SPP.CONCLUSION:These results suggested that SPP could be exploited and utilized as an approach of biological responsive modifier therapy in the treatment of acute leukemia.
ABSTRACT
The paper analyzes the new theory of “a modern property right system” put forward for the first time at the Third Plenary Session of the Sixteenth Central Committee of the CPC. It dwells on the opportunities and challenges facing state owned hospitals in establishing a modern hospital system. It is suggested that domestic hospitals should adopt a modern hospital system that fits in with the modern market economy and embodies modern property right features.
ABSTRACT
OBJECTIVE:To investigate the situation of the non-rational use of drugs in the outpatient and emergency department.METHODS:The prescriptions of outpatient and emergency department between Jan.2004 and Sept.2005 collected from Guilin Municipal Hospital of TCM were analyzed statistically.RESULTS:The irrational prescriptions represented 0.44% of the total prescriptions examined and verified.The non-rational use of drugs mainly manifested as improper usage,repeatedly administration,non-rational dosage,improper use of dissolvent,pharmacological antagonism and incompatibility etc.CONCLUSION:Some effective measures such as strengthening training of pharmacists,promoting the communication between pharmacists and physicians,developing careful work style etc should be taken to tackle the problem of non-rational use of drugs.
ABSTRACT
Objective To study the homocysteine induced gene(HCY-2) expression in human fetus arterial smooth muscle cells(hASMCs) in culture and explore the effects of homocysteine(HCY) and folic acid on HCY-2 expression and cell proliferation of hASMCs. Methods Immunohistochemistry ABC staining method was used to observe and analyze HCY-2 expression in hASMCs in culture.The image analysis system was used to research of hASMCs quantificationally.The effect of different HCY concentration on the proliferation of hASMCs was investigated by the cell counting. Results Immunoreactive substance of HCY-2 was chiefly found in cytoplasm of hASMCs.The expression of HCY-2 could be affected by HCY concentrations.There was a positive dose-dependent correlation with HCY concentrations in the culture medium.Folic acid increased the expression of HCY-2.The different concentration of HCY enhanced the proliferation of hASMCs,and this enhancement was maximal at the concentration of 1.25 mmol/L of HCY,while the proliferation was decreased when the concentration of HCY was over 1.25 mmol/L.Conclusion HCY increases the expression of HCY-2,and affects the proliferation of hASMCs.HCY is inhibited by folic acid.
ABSTRACT
This paper reported a rapid hydrolysis method of food protein using microwave oven and high pressure vessel technique. Tryptophan in hydroly-zate was determined by fluorimetric spectroscopy method. The hydrolytic agent was 5 M NaOH solution. Under the operative condition of the microwave oven (microwave output power 650W, 2 minutes 15 seconds and 65W 2 minutes) the highest tryptophan contents of the pure protein lysozymum and food SRM liver, cabbage and wheat flour were obtained. The results of the t-test showed that there was no significant difference between the tryptophan contents determined by using the operative condition of the microwave oven mentioned above and 145℃ 4h hydrolysis method. The recovery of the method was 93.3%-107%, and the coefficient of variation obtained by parallel determination of 10 lysozymum sample was 4.2%.
ABSTRACT
Objective To study the expression of homocysteine induced gene(HCY-2) in the normal and abnormal human embryonic heart. To research the roles of HCY-2 expressing protein in the human embryonic ventricular myocardial cell development and differentiation. Methods Immunohistochemistry and the technique of picture assay were used to detect HCY-2 expression in the normal and abnormal human embryonic heart. Results HCY-2 expresses during the development of human embryonic heart and mainly in the myocardial cells.The expression increases in the congenital malformation.Conclusion HCY-2 expression protein affects the development of human embryonic heart through affecting the myocardial cells proliferation and the abnormal expression has close relation to the congenital malformation.